Recently, the Lanzhou Food and Drug Administration Institute of Chinese medicine researcher published a paper designed to establish bear bile in ursodeoxycholic acid, chenodeoxycholic acid, cholic acid and deoxycholic acid test thin layer chromatography. It is pointed out that the method established in this paper is simple, rapid, sensitive and low cost, and can be used to screen a large number of samples. The article published in the year 2015, "Gansu Medical" magazine.
The supernatant of isooctane-diethyl ether-n-butanol-glacial acetic acid-water (20:10: 9: 10: 3) was used as the developing solvent. The solution was prepared by hydrolysis extraction method. With 10% sulfuric acid as a color reagent, in the UV lamp (365nm) under the view.
The method of specificity, high sensitivity, can be used for bear bile in ursodeoxycholic acid, chenodeoxycholic acid, cholic acid and pig deoxycholic acid check.