The rapid determination of the concentrations of chenodeoxycholic acid(CDCA),TUDCA, TCDCA and UDCA during the hydrolysis process

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The quantitative calibration models were established with the collected NIR spectra and the reference concentrations of chenodeoxycholic acid (CDCA) , tauroursodeoxycholic acid (TUDCA), taurochenodeoxycholic acid (TCDCA) and ursodeoxycholic acid (UDCA) using partial least squares regression algorithm. The samples of new batches can be determined rapidly after the models were established and validated, and the hydrolysis process of bear bile powder can be monitored quantitatively. Additionally, a moving block of standard deviation (MBSD) method was also developed for the endpoint determination of the hydrolysis process. The proposed methods have reduced the laborious workload of process sample analysis significantly, and the fast analytical results have contributed to the understanding and controlling of the bear bile powder hydrolysis process.A near infrared (NIR) spectroscopy-based method was developed for monitoring the hydrolysis process of bear bile powder. During the hydrolysis process, samples were collected and measured using both NIR spectrometer and high performance liquid chromatography. In summary, samples were measured by NIR spectrometer and high performance liquid chromatography.
In this research, an NIRS based method was established for the rapid determination of the concentrations of chenodeoxycholic acid (CDCA), TUDCA, TCDCA and UDCA during the hydrolysis process, and on this basis, a moving block of standard deviation (MBSD) method for the endpoint determination was also proposed. To our best knowledge, this is the first report on the NIRS application in the hydrolysis process monitoring of animal origin TCMs, which is a common challenge in the Chinese pharmaceutical industry, therefore, it is a very important research. The presented method may also provides a promising tool for solving the similar problems.
The contents of chenodeoxycholic acid(CDCA), TUDCA, TCDCA and UDCA in the samples are determined using a validated HPLC-ELSD method as the reference method. For HPLC measurements, an Agilent 1100 HPLC system (Agilent Technologies, Santa Clara, CA, USA) with a vacuum degasser, a quaternary pump, an autosampler, a thermostatic column compartment, and an evaporative light scattering detector (ELSD) were used. The deep-going analysis method should also be adoped in furture.