Study on the antitussive effect of chenodeoxycholine acid:
Effects of ammonia on cough in mice: the use of ammonia stimulation. Forty mice were randomly divided into 4 groups (n = 10). Distilled water group given 10ml /kg of distilled water, the other groups according to Table 1 dose of gavage given the corresponding liquid. Mice were placed in a bell jar 2 h after administration and stimulated with 1 ml of concentrated aqueous ammonia (25.0%). After 20 s stimulation, the mice were quickly removed and the cough latency was measured and the mice the number of coughs.
The results showed that low dose and high dose of chenodeoxycholine acid had a strong antitussive effect on ammonia-induced cough, and there was no significant difference between high and low dose groups.
Effects of sulfur dioxide on cough in mice: the use of sulfur dioxide stimulation. First, sodium sulphite and concentrated sulfuric acid into the gas generator, the gas generator outlet connected to a three-way tube, the other two mouth of the three tubes were followed by a balloon and syringe, extraction of quantitative sulfur dioxide gas to stimulate mice. Forty mice were divided into 4 groups (n = 10). Mice were placed in 250 ml jar 2 h after administration and the incubation period of mice cough was observed by quantitative injection of sulfur dioxide gas.
The results showed that low dose and high dose of chenodeoxycholine acid had a strong antitussive effect on sulfur dioxide-induced mouse cough.