The chemical composition, pharmacological effects, clinical application and the resource status of wild black bear in China and the development of artificial bear industry were reviewed. The properties of ursodeoxycholic acid and chenodeoxycholic acid were studied, and the HPLC chromatographic conditions were improved to solve the problem that the bile acid components reflected in the previous studies were easy to dissociate and the UV absorption was weak and the resulting peak profile is not good, tailing serious, resulting in the composition of the bear bile and the content of the problem is not accurate. The results showed that the content of ursodeoxycholic acid and chenodeoxycholic acid in the samples were analyzed. The effects of ursodeoxycholic acid and chenodeoxycholic acid content of the annual change in the law. The results showed that the content of the two components was low, which was 30.11% ± 2.8% and 42.47% ± 2.88%, 32.02% ± 2.26%, respectively. The content of the two components was low, And 38.28% ± 1.43%, 33.06% ± 2.06% and 38.83% ± 5.37% respectively, and fluctuated greatly in the whole year, which showed the regularity of summer and autumn. The study shows that this change is closely related to the climate and environment of the bear market, the formula of diets, the way of bile preparation, the bile processing method and the drainage technique. This research is of great significance to improve the quality and yield of the products and improve the economic benefits.