Cyanodeoxycholic acid nitrogen derivatives can inhibit the proliferation of a variety of cancer cells and can induce a variety of cancer cell apoptosis. From the chenodeoxycholic acid starting, after esterification, Jones reagent oxidation, reduction and other reactions, respectively, in the 3-position or 7-position of the chenodeoxycholic acid nucleus introduction of oxime, methoximime, benzyloxime and nitrogen-containing groups such as thiosemicarbazone with different substituents. The structures were characterized by IR, NMR and Ms and other modern analytical methods. (SG-7901), human hepatocellular carcinoma cells (Bel-7404) and prostate cancer cells (PC-3) were used to study the proliferation and proliferation of tumor cells in vitro. The results showed that the growth and proliferation of tumor cells were inhibited by human gastric cancer cells (SGC-7901). Some chenodeoxycholic acid nitrogen compounds have a good inhibitory effect on prostate cancer cells (PC-3) and human hepatoma cells(Bel7404).
Chenodeoxycholic acid and 1,25- (OH) <2> D <3> on the growth and differentiation of leukemia HL-60 cells were studied by MTT assay and flow cytometry. Methods: Cell growth inhibition was measured by MTT assay. Apoptosis, differentiation and cycle of cells were observed by flow cytometry. Results: The growth and differentiation of HL-60 cells were only treated with chenodeoxycholic acid (60μmol / L) and 1,25- (OH) <2> D <3> (1nmol / L, 5nmol / L) and the effect of the two drugs on the growth of HL-60 cells was significantly higher than that of the two drugs (the difference between the two groups was significant P <0.05), and at different time points, the third day was more obvious, the fifth day of the most obvious (the difference was significant P <0.05), drug combination in the role. Objective: To study the effects of chenodeoxycholic acid (CDCA) and 1,25- (OH) <2> D <3> on the growth and differentiation of leukemia HL-60 cells by MTT assay and flow cytometry Its mechanism. Methods: Cell growth inhibition was measured by MTT assay. Apoptosis, differentiation and cycle of cells were observed by flow cytometry. Results: The growth and differentiation of HL-60 cells were only treated with chenodeoxycholic acid (60μmol / L) and 1,25- (OH) <2> D <3> (1nmol / L, 5nmol / L) and the effect of the two drugs on the growth of HL-60 cells was significantly higher than that of the two drugs (the difference between the two groups was significant P <0.05), and at different time points, the third (P <0.05). The combination of drugs at 72 hours could induce about 73% of HL-60 cells to differentiate into mature monocytes (the difference between them was significant (P <0.05)), and the difference was significant (P <0.05), but no significant effect of apoptosis.