Yourlocation: Home > News > Simultaneous Determination of Chenodeoxycholic Acid and Deoxycholic Acid by High Performance Liquid Chromatography-Evaporative Light Scattering Detector
A high performance liquid chromatography - evaporative light scattering detector (HPLC - ELSD) method for the determination of porcine deoxycholic acid and chenodeoxycholic acid in bile betelweave B1 capsules was established. Method: Thermo U -3000 liquid chromatography system, ChromeleonTM 7 workstation; ELSD 2000ES detector, drift tube temperature of 105 ℃, impact tube closed, the gain of 1; gas flow rate of 2.0 L / min. The mobile phase consisted of acetonitrile - 0.1% glacial acetic acid (53:47) at a flow rate of 1.0 mL / min and a column temperature of 25 °C using a Thermo C18 column (250 mm × 4.6 mm, 5 μm). Results: The concentration of deoxycholic acid was 21.53 ~ 258.36μg / mL (r = 0.9997), and the linear relationship between chenodeoxycholic acid and 11.148 ~ 133.776μg / mL (r = 0.9995) The average recoveries of goose were 101.07% and RSD = 2.49% (n = 9). The average recoveries of chenodeoxycholic acid were 97.10% and RSD = 2.54% (n = 9). Conclusion: The method is simple, accurate, stable and sensitive, and can be used for the quality control of bile betelweave B1 capsule.
HPLC-ELSD method for simultaneous determination of deoxycholic acid and chenodeoxycholic acid in Hugan Tablets by high performance liquid-evaporative light scattering assay. Methods: Agilent Zorbax SB-C18 (4.6 mm × 250 mm, 5 μm) column was used. The mobile phase consisted of methanol-0.2% glacial acetic acid solution (80:20) at a flow rate of 1.0 m L · min-1. 30 ℃; Evaporative light detector carrier gas for nitrogen, flow rate of 2.0 L · min-1, drift tube temperature of 60 ℃. Results: The linear regression was 97.41% and the RSD was 1.30% (n = 6) in the range of 0.521 ~ 10.42μg (r = 0.999 9), and the yield of chenodeoxycholic acid was (R = 0.999 4). The average recoveries were 96.09% and RSD was 2.11% (n = 6). The results showed that the average recoveries were in the range of 0.266 ~ 5.32μg. Conclusion: The method is accurate, simple and sensitive, and it can be used for the quality control of pig milk powder in Hugan Tablets.

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