Yourlocation: Home > News > Research two-step reaction from the chenodeoxycholic acid to UDCA
Methods: Twenty-four healthy male Wistar rats were randomly divided into three groups (n=8): normal control group, high fructose group and chenodeoxycholic acid group. Rats were sacrificed by the end of 16 weeks after feeding. Blood urea nitrogen, serum creatinine, fast glucose, lipid concentration were observed, spot urine samples were obtained to measure the albumin and creatinine levels. Triglyceride of renal cortices was detected. The mRNA level and protein contents of the fibrosis-inducing growth factor transforming growth factor β1 (TGF-β1) and plasminogen activator inhibitor (PAI-I), inflammatory cytokines tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), oxidative stress index NADPH oxidase 2 (Nox2) and p22phox in kidney were examined. The pathological changes of kidney were examined by PAS staining and immunohistochemical staining. Electron microscope sections were made to measure glomerular basement membrane (GBM) width. 
Results: Renal injuries including mesangial expansion, GBM thickness and podocyte foot process effacement were found in fructose-fed Wistar rats, FXR agonist chenodeoxycholic acid modulates renal lipid metabolism, decreases proteinuria and improves renal fibrosis, inflammation and oxidation stress. High-fructose-feeding may cause lipid nephrotoxicity through down-regulated farnesoid X receptor and increases expression of profibrotic growth factors, proinflammatory cytokines, and oxidative stress in Wistar rats. Conclusion: FXR activation by chenodeoxycholic acid can prevent the injury in kidney induced by high fructose feeding.
Research two-step reaction from the chenodeoxycholic acid to UDCA, which is to produce an intermediate product 7-keto lithocholic acid by oxidation of chenodeoxycholic acid, and then the asymmetric reduction to give 7-keto lithocholic bear. The first step for the electrochemical oxidation reaction is to Br- / Br2 as a medium, electrolysis is generated Br2 oxidant CDCA 7 hydroxyl, the product is 7-keto lithocholic acid, Br2 Br is reduced to re-electrode the oxidation can be realized in the Br2 recycling. Study on ruthenium titanium, graphite, titanium-based lead dioxide electrode cyclic voltammetry three kinds of behavior in the absence of diaphragm electrolyzer and H- diaphragm cell electrolysis system, the analysis confirms the cyclic voltammetry of CDCA indirect Electrochemical oxidation process.

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