Background: Recent studies indicate farnesoid X receptor (FXR) plays an important role in regulating lipid metabolism in kidney disease. The purpose of the present study is to investigate the effect of chenodeoxycholic acid (CDCA), a FXR agonist, on fibrosis, inflammation and oxidative stress in kidney in rats fed on high fructose.
Hydrophobicity is the most important determinant of toxicity of bile acids (BAs) and depends on the number, position and orientation of hydroxyl groups. Ursodeoxycholic acid (UDCA) is a hydrophilic dihydroxy BA, which is formed by 7β-epimerization of chenodeoxycholic acid (CDCA) in the gut by intestinal bacteria. Unlike the other secondary BAs, UDCA exerts antiapoptotic effects by preventing oxidative stress. The aim of our study was to analyze the influence of stereochemistry of hydroxyl groups on antiproliferative activity of BAs.
Compared of ursodeoxycholic acid and chenodeoxycholic acid, from the structural relationship, application of measuring method, preparation, research and other clinical progress made comprehensive analysis were studied. Extract bilirubin pig powder was used as raw material, the reaction temperature, pH, CaCl2 dosage, reaction time effect on the CDCA purity were investigate, experiments show that: the reaction temperature was 45 ℃, the sample pH was 10.5, CaCl2 dosage was 10% of the mass of the sample, the reaction time was 2h, chenodeoxycholic acid purity was 47.05%.