Improved synthesis of 6-α ethoxy chenodeoxycholic acid. Methods: The target compound 6-α ethoxy deoxycholic acid was obtained by oxidizing, esterifying, etherifying, reducing and hydrogenating with chenodeoxycholic acid as the starting material. Results: The total yield of the target compound was 42%, and its structure was confirmed by ~ 1H NMR and MS. Conclusion: By improving the reaction process to simplify the operation, the yield has improved, with a high practical value.
To study the effect of synthetic deoxycholic acid derivative HS-1200 on the proliferation of hepatic stellate cells and the synthesis of type I collagen in vitro. Methods: HSC-T6 cells were stimulated with 5ng / ml TGF-β1 and then HS-1200 (60μM, 40μM, 20μM) with different concentration gradient was administered at 24h and 48h. In this study, cell proliferation was measured by MTT assay, and cell cycle was measured by flow cytometry. The expression of type I collagen, cyclin-D1 and cyclin-E was studied by immunohistochemistry. Apoptosis was studied by DNA fragment analysis. Results: This study found that HS-1200 inhibited the proliferation of HSC induced by TGF-β1 and captured the cells in the G0 /G1 phase of the cell cycle. Conclusion: HS-1200 inhibits HSC-T6 proliferation and type I collagen synthesis, and has the potential as a clinical anti-fibrosis agent.