Yourlocation: Home > News > Effects of pH and CaCl2 addition on chenodeoxycholic acid purity
Effect of sample pH on chenodeoxycholic acid(CDCA) purity:
Accurately weighed 5 copies of 10g bile powder plus 50mL distilled water, with 15% NaOH were the sample pH adjusted to 8.5,9.5,10.5,11.5,12.55 level, CaCl2 addition of 10%, 50 ℃ water bath 1h, and then according to the experiment, the program was washed with water, adjusted to pH and dried. The content of chenodeoxycholic acid(CDCA) in bile powder was measured and the relationship between pH and CDCA purity was plotted based on the obtained data.
It can be seen from Fig.1 that the purity of chenodeoxycholic acid(CDCA) is significantly increased with the increase of pH (p <0.05), CDCA purity is the highest at pH 10.5, and CDCD purity is significantly decreased (p <0.05) with increasing pH. This is due to the greater solubility of cholic acid in alkaline conditions, with the increase in pH solubility gradually increased, but after the pH reached 10.5 after the bile acid molecules were destroyed to reduce cholic acid content. Thus 9.5, 10.5, and 11.5 are the optimum pH for extracting CDCA.
Five parts of 10 g of bile powder and 50 mL of distilled water were accurately weighed, adjusted to pH 10.5 with 15% NaOH, and 6%, 8%, 10%, 12% and 14% of CaCl2 were added and the water was treated at 50 °C for 1h, and then according to the experimental program for washing, adjusting the pH and drying treatment. The content of CDCA in bile powder was measured and the relationship between CaCl2 addition and CDCA purity was plotted.
The purity of CDCA was the highest at 10%, and the purity of chenodeoxycholic acid(CDCA) was higher than that of 12% (p> 0.05). Thus, when adding 8%, 10%, 12%, the optimal amount of CaCl2 extracted for CDCA.

Address:A3 Building, Dongli Aviation Business District,No.8,Pingying Road, Dongli District, Tianjin, P.R.China, 300300 Tel:+86-022-58602231 Fax:+86-022-58602232
Copyright © Tianjin NWS Biotechnology and Medicine Co. Ltd.