In this study, we investigated the effect of cholecystectomy on the expression and activity of alkaline sphingomyelinase (Alk-SMase) and the effect of ursodeoxycholic acid (UDCA) in the cholecystectomy model. Methods: The mouse cholecystectomy  expression level and activity of Alk-SMase protein were detected by protein blotting and liquid scintillation counting. Results: Compared with the control group, the expression of Alk-SMase protein in the feces of mice was detected by liquid chromatography. Removal of the gallbladder in mice cholic acid (CA) and chenodeoxycholic acid (CDCA) levels decreased; elevated deoxycholic acid (DCA) level; and lithocholic acid (LCA) levels did not change on removal of the gallbladder. After oral intervention UDCA, CDCA and CA cholecystectomy elevated levels in mice, and decreased levels of DCA; LCA levels did not change after the removal of the gall bladder, the intestine of mice was Alk-SMase. The expression levels and activity of the protein as compared to the control group significantly decreased sexual; use of UDCA in mice after cholecystectomy intervened, Alk-SMase protein expression level and the activity was significantly higher No treatment group. Conclusion: Oral UDCA intervention cholecystectomy can effectively prevent feces caused cholecystectomy bile acid content and the level of expression of Alk-SMase protein in intestinal tissue and changes in activity.