Yourlocation: Home > News > Cystic acid (CA) and chenodeoxycholic acid (CDCA) levels were reduced in mice with cholecystectomy
In this study, we investigated the effect of cholecystectomy on the expression and activity of alkaline sphingomyelinase (Alk-SMase) and the effect of ursodeoxycholic acid (UDCA) in the cholecystectomy model. Methods: The mouse cholecystectomy  expression level and activity of Alk-SMase protein were detected by protein blotting and liquid scintillation counting. Results: Compared with the control group, the expression of Alk-SMase protein in the feces of mice was detected by liquid chromatography. Removal of the gallbladder in mice cholic acid (CA) and chenodeoxycholic acid (CDCA) levels decreased; elevated deoxycholic acid (DCA) level; and lithocholic acid (LCA) levels did not change on removal of the gallbladder. After oral intervention UDCA, CDCA and CA cholecystectomy elevated levels in mice, and decreased levels of DCA; LCA levels did not change after the removal of the gall bladder, the intestine of mice was Alk-SMase. The expression levels and activity of the protein as compared to the control group significantly decreased sexual; use of UDCA in mice after cholecystectomy intervened, Alk-SMase protein expression level and the activity was significantly higher No treatment group. Conclusion: Oral UDCA intervention cholecystectomy can effectively prevent feces caused cholecystectomy bile acid content and the level of expression of Alk-SMase protein in intestinal tissue and changes in activity.

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