The results showed that there were 18: 1 LPC, 18: 2 LPC, 18: 0 LPC, 16: 0 LPC, cholic acid, Cer (d18: 1/23: 0), and the potential biomarkers related to type 2 diabetes mellitus ), Phenylalanine, tryptophan; the potential biomarkers in the urine are 3-indol phenol sulfuric acid, hippuric acid, N-acetyl-L-tyrosine, indole-3-carboxylic acid, acid, melatonin, alachlor, cholic acid, 4-O-Methylmelledide, valine, phenylalanine, tryptophan; fecal potential biomarkers are 7-oxocholic acid, 7-a -Hydroxxy-3-oxo-5b-cholanoic acid, hyaluronic acid, ursodeoxycholic acid, chenodeoxycholic acid, deoxycholic acid, cholic acid, 7-oxodeoxycholic acid, 3-oxocholic acid, Valine, phenylalanine. Diabetes-related metabolic pathways are: phenylalanine, tryptophan and tyrosine biosynthesis; phenylalanine metabolism; tryptophan metabolism; bile acid biosynthesis; glycerophospholipid metabolism. Statistical analysis showed that the contents of most biomarkers were significantly reduced to the blank group after administration of total coumarin. Conclusion: The study of diabetes mellitus related biomarkers and metabolic pathways, the initial exploration of the pathogenesis of type 2 diabetes and the body of amino acids, lipid metabolism. And found that high doses of total Hu total coumarin has a better role in the treatment of type 2 diabetes, its efficacy and Xiaoke pills quite. To explore the mechanism of action may be to regulate lipid metabolism, amino acid synthesis and metabolism, promote insulin secretion, enhance the body's sensitivity to insulin, regulate intestinal metabolic metabolism, reduce the complications of diabetes.