Chenodeoxycholic acid expectorant effect study: the use of tracheal phenol red method, take 40 mice, were randomly divided into 4 groups, each group of 10, group, dose, route of administration was the same. After 1 h of administration, the mice were intraperitoneally injected with phenol red saline at 015 g /kg, and the mice were sacrificed at 15 h, and the connective tissue around the trachea was carefully removed. A trachea was cut off from the thyroid cartilage to the branch of the bronchus. Into a tube containing 2 ml of physiological saline, add 1mol /L sodium hydroxide 11ml, shake, and then use ultraviolet spectrophotometer at the wavelength of 546nm measured A value.
The results showed that both the high dose and the low dose could increase the secretion of phenol red in the trachea of mice, and there was no significant difference between high and low dose.
Cough, sputum, asthma is the main symptoms of acute and chronic non-specific inflammation of the respiratory tract, in the inflammatory process, eosinophils, lymphocytes, macrophages and other inflammatory cells to synthesize and release a variety of inflammatory mediators, causing respiratory inflammation. Inflammatory cells release inflammatory mediators play an regulatory role in the function of inflammatory cells, the conduction of cell information, and the growth, proliferation and differentiation of cells. Chenodeoxycholic acid antitussive, expectorant and asthma test results show that chenodeoxycholic acid has a strong antitussive, expectorant and asthma effect. Its mechanism of action through the impact of the body's inflammatory mediators or inflammatory cells to achieve the function needs further study.