Yourlocation: Home > News > Chenodeoxycholic Acid Reduces Hypoxia Inducible Factor-1α Protein and Its Target Genes
This study evaluated HIF-1α inhibitors under different hypoxic conditions, physiological hypoxia (5% O2) and severe hypoxia (0.1% O2). We found that chenodeoxycholic Acid(CDCA) reduced the amount of HIF-1α protein only under physiological hypoxia but not under severe hypoxia without decreasing its mRNA level. By using a proteasome inhibitor MG132 and a translation inhibitor cyclohexamide, we showed that CDCA reduced HIF-1α protein by decreasing its translation but not by enhancing its degradation. The following findings indicated that farnesoid X receptor (FXR), a CDCA receptor and its target gene, Small heterodimer partner (SHP) are not involved in this effect of CDCA. 
Distinctly from chenodeoxycholic Acid, MG132 prevented SHP and an exogenous FXR agonist, GW4064 from reducing HIF-1α protein. Furthermore a FXR antagonist, guggulsterone failed to prevent CDCA from decreasing HIF-1α protein. Furthermore, guggulsterone by itself reduced HIF-1α protein even in the presence of MG132. These findings suggested that CDCA and guggulsterone reduced the translation of HIF-1α in a mechanism which FXR and SHP are not involved. This study reveals novel therapeutic functions of traditional nontoxic drugs, CDCA and guggulsterone, as inhibitors of HIF-1α protein.
Calcium chloride instead of the traditional barium chloride, extracted from bilirubin pig paste waste separation and purification of crude chenodeoxycholic acid, and then use different PH value, acid and other hydrophilic impurities Chenodeoxy acid have different solubility in dilute aqueous ammonia, with dilute ammonia as leather fetch agent,  purified to obtain CDCA boutique.

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